BMPR-II Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

BMPR-II Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BMPR-II
  Ala26-Ile151
  Accession # Q13873
• Specificity: Detects human BMPR‑II in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) BMPR-IA and rhBMPR-1B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BMPR2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMPR-II Antibody [Biotin]

• BMP type II receptor
• BMP type-2 receptor
• BMPR2
• BMPR-2
• BMPR3
• BMPRII
• BMPR-II
• BMPR-IIprimary pulmonary hypertension 1
• Bone morphogenetic protein receptor type II
• bone morphogenetic protein receptor type-2
• bone morphogenetic protein receptor, type II (serine/threonine kinase)
• BRK-3
• EC 2.7.11
• EC 2.7.11.30
• FLJ41585
• FLJ76945
• PPH1BMR2
• T-ALK
• type II activin receptor-like kinase
• type II receptor for bone morphogenetic protein-4

Background

Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signl transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4 and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity.

1. Rosenzweig, B.L. et al. (1995) Proc. Natl. Acad. Sci. USA 92:7632.
2. Beppu, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:499.
3. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMPR-II Antibody (BAF811)(5)

Publications using BAF811

• LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5 PLoS ONE, 2017-05-10;12(5):e0177188. 2017-05-10 [PMID: 28489883] (Flow Cytometry, Human)
• Huse K, Bakkebo M, Walchli S, Oksvold M, Hilden V, Forfang L, Bredahl M, Liestol K, Alizadeh A, Smeland E, Myklebust J Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma. PLoS ONE, 2012-10-01;7(10):e46117. 2012-10-01 [PMID: 23049692] (Flow Cytometry, Human)
• Huse K, Bakkebo M, Oksvold MP, Forfang L, Hilden VI, Stokke T, Smeland EB, Myklebust JH Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: differential effects of BMP-6 and BMP-7. Eur. J. Immunol., 2011-10-18;41(11):3135-45. 2011-10-18 [PMID: 21898381] (Flow Cytometry, Human)
• Sivertsen EA, Huse K, Hystad ME, Kersten C, Smeland EB, Myklebust JH Inhibitory effects and target genes of bone morphogenetic protein 6 in Jurkat TAg cells. Eur. J. Immunol., 2007-10-01;37(10):2937-48. 2007-10-01 [PMID: 17899540] (Flow Cytometry, Human)
• Kersten C, Dosen G, Myklebust JH, Sivertsen EA, Hystad ME, Smeland EB, Rian E BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3. Exp. Hematol., 2006-01-01;34(1):72-81. 2006-01-01 [PMID: 16413393] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: BMPR2
• Uniprot:
  • Q13873()