Recombinant Human BMPR-IA/ALK-3 Protein

Bioactivity: Recombinant Human BMPR-IA/ALK-3 Protein [NBP2-76260] - BMPR-IA/ALK-3 induced inhibition of BMP-4 activity on ATDC5 cells.

Product Discontinued

Recombinant Human BMPR-IA/ALK-3 Protein Summary

• Description: For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: BMPR-IA/ALK-3 is a dimer, 40.4/80.8 kDa (362/724 aa)
• Details of Functionality: Inhibition of human BMP-4-induced alkaline phosphatase production by ATDC5 cells, >8.3 x 10^3 units/mg (ED50 acceptance criteria are <120 ng/mL).
• Source: CHO
• Protein/Peptide Type: Recombinant Protein
• Gene: BMPR1A
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 80.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing phosphate buffered saline (PBS)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Alternate Names for Recombinant Human BMPR-IA/ALK-3 Protein

• ACVRLK310q23del
• ALK-3
• ALK3EC 2.7.11.30
• BMP type-1A receptor
• BMPR1A
• BMPR-1A
• BMPRIA
• BMPR-IA
• bone morphogenetic protein receptor type-1A
• bone morphogenetic protein receptor, type IA
• CD292 antigen
• CD292
• EC 2.7.11
• Serine/threonine-protein kinase receptor R5
• SKR5
• type II-like kinase 3

Background

The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BMPR1A