BMAL1 Antibody (OTI1C11)

Western Blot: BMAL1 Antibody (OTI1C11) [NBP2-02544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BMAL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMAL1.

Immunocytochemistry/Immunofluorescence: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNTL .

Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-BMAL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue N93ing anti-BMAL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded colon tissue using anti-BMAL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded lung tissue using anti-BMAL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded pancreas tissue using anti-BMAL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded prostate tissue using anti-BMAL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BMAL1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BMAL1 Antibody (OTI1C11) [NBP2-02544] - Staining of paraffin-embedded bladder tissue using anti-BMAL1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI1C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

BMAL1 Antibody (OTI1C11) Summary

• Description: Novus Biologicals Mouse BMAL1 Antibody (OTI1C11) (NBP2-02544) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-BMAL1 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ARNTL(NP_001169) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMAL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID: 31300350).
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 68.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31300350). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BMAL1 Antibody (OTI1C11)

• ARNTL
• aryl hydrocarbon receptor nuclear translocator-like protein 1
• aryl hydrocarbon receptor nuclear translocator-like
• basic-helix-loop-helix-PAS orphan MOP3
• BHLHE5
• bHLHe5brain and muscle
• bHLH-PAS protein JAP3
• BMAL1
• BMAL1c
• BMAL1TIC
• Brain and muscle ARNT-like 1
• Class E basic helix-loop-helix protein 5
• JAP3
• Member of PAS protein 3
• member of PAS superfamily 3
• MOP3
• MOP3BMAL1c
• PAS domain-containing protein 3
• PASD3
• PASD3MGC47515
• TIC

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMAL1 Antibody (NBP2-02544)(6)

Publications using NBP2-02544

• Liu T, Wang Z, Ye L et al. Nucleus-exported CLOCK acetylates PRPS to promote de novo nucleotide synthesis and liver tumour growth Nature cell biology 2023-01-16 [PMID: 36646788] (WB, Human)
• Vorgelegt V, Wen W Expression and function of blood receptors ROR?, REVERBA and BMAL1 in human colorectal cancer Thesis 2022-01-01 (IHC)
• Kaneshiro K, Nakagawa K, Tsukamoto H et al. The clock gene Bmal1 controls inflammatory mediators in rheumatoid arthritis fibroblast-like synoviocytes Biochemical and biophysical research communications 2023-11-24 [PMID: 38043198]
• He M, Li L, Li J et al. rs2253820 Variant Controls Blood Pressure Dip After Stroke by Increasing CLOCK-BMAL1 Expression Translational stroke research 2022-07-23 [PMID: 35870088] (IHC-P, WB, Rat)
• Aroca-Siendones MI, Moreno-SanJuan S, Puentes-Pardo JD Et al. Core Circadian Clock Proteins as Biomarkers of Progression in Colorectal Cancer Biomedicines 2021-08-06 [PMID: 34440171] (IHC-P, Human)
• Burgermeister E, Battaglin F, Eladly F et al. Aryl hydrocarbon receptor nuclear translocator-like (ARNTL/BMAL1) is associated with bevacizumab resistance in colorectal cancer via regulation of vascular endothelial growth factor A EBioMedicine 2019-07-01 [PMID: 31300350] (IHC-Fr, WB, Mouse)

Bioinformatics

• Gene Symbol: BMAL1
• Entrez:
  • 406(Human)