BLTP2 Antibody (A0-F3-R)

Western blot analysis of BLTP2 on different lysates with BLTP2 Antibody (A0-F3-R) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: A431 cell lysate Lane 6: PC-3M cell lysate Lane 7: LNCaP cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 254 kDa Observed band size: 55 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32500) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Immunohistochemistry: BLTP2 Antibody (A0-F3-R) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry analysis of HeLa cells labeling K0100 with BLTP2 Antibody (A0-F3-R) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with BLTP2 Antibody (A0-F3-R) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: A0-F3-R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

BLTP2 Antibody (A0-F3-R) Summary

• Description: Novus Biologicals Mouse BLTP2 Antibody (A0-F3-R) (NBP3-32500) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human BLTP2 aa 1,947-2,235 / 2,235. (Uniprot: Q14667)
• Localization: Cell membrane, Endoplasmic reticulum membrane, Mitochondrion membrane.
• Source: HEK293
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BLTP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:1000
• Theoretical MW: 254 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BLTP2 Antibody (A0-F3-R)

• Antigen MLAA-22
• BCOX
• BCOX1
• breast cancer overexpressed gene 1
• Breast cancer-overexpressed gene 1 protein
• bridge-like lipid transfer protein family member 2
• cancer/testis antigen 101
• CT101
• DKFZp686M0843
• FMP27
• Hob
• hypothetical protein LOC9703
• KIAA0100
• MGC111488
• MGC134981
• protein hobbit homolog
• U937-associated antigen

Background

This gene was initially characterized in human as having high expression levels in breast carcinomas and breast cancer cell lines. This gene also has increased expression in prostrate cancer cells relative to normal prostrate tissues. Expression of this gene is negatively regulated by direct binding of the microRNA miR-195 to its 3' UTR. miR-195 has been shown to modulate the invasiveness of prostrate cancer cells and xenograft metastases by downgrading expression of this gene. In mouse, the protein encoded by this gene was identified as an antigen on acute monocytic leukemia cells. In human, alternative splicing results in multiple transcript variants encoding distinct isoforms; some of these isoforms are predicted to contain an RNA pol II promoter FMP27 protein domain and a Golgi-body-localization APT1 domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BLTP2