Recombinant Human BLNK His Protein

SDS-Page: BLNK Protein [NBP1-50993]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human BLNK His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-456 of Human BLNK
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BLNK
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 52.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human BLNK His Protein

• AGM4
• B cell linker protein
• BASH
• B-cell adapter containing a SH2 domain protein
• B-cell adapter containing a Src homology 2 domain protein
• B-cell linker protein
• B-cell linker
• BLNK
• BLNK-s
• Cytoplasmic adapter protein
• Ly57
• MGC111051
• SLP65
• SLP-65BLNK-S
• SLP65Ly57
• Src homology 2 domain-containing leukocyte protein of 65 kDa

Background

BLNK, also known as B-cell linker, is essential for normal B-cell development. This protein has been shown to associate with the effector proteins GRB2, Vav, NCK and PLC gamma following activation of the B cell receptor. It is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLC gamma. Recombinant human BLNK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BLNK