BLCAP Antibody (4E1)

Western Blot: BLCAP Antibody (4E1) [NBP2-75420] - Analysis of BLCAP on recombinant protein tissue lysate using anti-BLCAP antibody at 1/5,000 dilution.

Immunocytochemistry/Immunofluorescence: BLCAP Antibody (4E1) [NBP2-75420] - Staining BLCAP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BLCAP Antibody (4E1) [NBP2-75420] - Analysis of paraffin-embedded rat cervix tissue using anti-BLCAP antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BLCAP Antibody (4E1) [NBP2-75420] - Analysis of paraffin-embedded human breast tissue using anti-BLCAP antibody. Counter stained with hematoxylin.

Product Discontinued

BLCAP Antibody (4E1) Summary

• Immunogen: Synthetic peptide within Human BLCAP aa 38-87 / 87. (SwissProt: P62952 Human; SwissProt: P62950 Rat)
• Localization: Membrane.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: BLCAP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for BLCAP Antibody (4E1)

• Bc10
• BC10Bladder cancer 10 kDa protein
• bladder cancer associated protein
• bladder cancer related protein (10kD)
• bladder cancer-associated protein

Background

BLCAP is encoded by this gene was identified using a differential display procedure with tumor biopsies obtained from a noninvasive and an invasive bladder transitional cell carcinoma. Although database searches revealed no homology to any human gene at the time of identification, mouse, rat and zebrafish orthologs have since been identified. The function of this differentially expressed protein is not yet known but it appears to be down-regulated during bladder cancer progression. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BLCAP