BIN3 Antibody (OTI8E9) - Azide and BSA Free

Western Blot: BIN3 Antibody (OTI8E9) - Azide and BSA Free [NBP2-72051] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIN3.

Immunocytochemistry/Immunofluorescence: BIN3 Antibody (OTI8E9) - Azide and BSA Free [NBP2-72051] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BIN3.

Immunohistochemistry: BIN3 Antibody (OTI8E9) - Azide and BSA Free [NBP2-72051] - Staining of paraffin-embedded Human prostate tissue using anti-BIN3 mouse monoclonal antibody.

Flow Cytometry: BIN3 Antibody (OTI8E9) - Azide and BSA Free [NBP2-72051] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-BIN3 antibody, and then analyzed by flow cytometry.

Western Blot: BIN3 Antibody (OTI8E9) - Azide and BSA Free [NBP2-72051] - Analysis of extracts (35ug) from 9 different cell lines by using anti-BIN3 monoclonal antibody.

Immunohistochemistry: BIN3 Antibody (OTI8E9) - Azide and BSA Free [NBP2-72051] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BIN3 mouse monoclonal antibody.

Immunohistochemistry: BIN3 Antibody (OTI8E9) - Azide and BSA Free [NBP2-72051] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BIN3 mouse monoclonal antibody.

Immunohistochemistry: BIN3 Antibody (OTI8E9) - Azide and BSA Free [NBP2-72051] - Staining of paraffin-embedded Human thyroid tissue using anti-BIN3 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI8E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

BIN3 Antibody (OTI8E9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse BIN3 Antibody (OTI8E9) - Azide and BSA Free (NBP2-03389) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human BIN3 (NP_061158) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: BIN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 29.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for BIN3 Antibody (OTI8E9) - Azide and BSA Free

• bridging integrator 3
• MGC14978

Background

BIN3, also known as Bridging integrator 3, is a 253 amino acid protein that plays a role in cell membrane shape and structure through interaction with GTPases, and members of the BAR domain protein family have been shown to be involved in processes such as intracellular transport and endocytosis. This protein is currently being studied for research on the following diseases and disorders, ornithosis, pancreatic cancer, and pancreatitis. Interactions with the BIN3 protein have been shown to involve DNMBP, GRPEL1, VDAC3, POR, and RAD23B, CAP2B, and ERH in cell cycle, actin filament organization, cytokinesis, protein localization, and barrier septum assembly pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BIN3