Biglycan Antibody (JB71-31)

Western Blot: Biglycan Antibody (JB71-31) [NBP2-75419] - Analysis of Biglycan on mouse heart and human skin tissue lysates using anti-Biglycan antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Biglycan Antibody (JB71-31) [NBP2-75419] - Analysis of paraffin-embedded mouse heart tissue using anti-Biglycan antibody. Counter stained with hematoxylin.

Flow Cytometry: Biglycan Antibody (JB71-31) [NBP2-75419] - Analysis of HepG2 cells with Biglycan antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: Biglycan Antibody (JB71-31) [NBP2-75419] - Analysis of paraffin-embedded human kidney tissue using anti-Biglycan antibody. Counter stained with hematoxylin.

Product Discontinued

Biglycan Antibody (JB71-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Biglycan aa 260-368 / 368. (SwissProt: P21810 Human; SwissProt: P28653 Mouse)
• Localization: Extracellular matrix. Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BGN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Biglycan Antibody (JB71-31)

• BGN
• Biglycan
• Bone/cartilage proteoglycan I
• bone/cartilage proteoglycan-I
• dermatan sulphate proteoglycan I
• DSPG1
• PGI
• PG-S1
• SLRR1A
• SLRR1Abiglycan proteoglycan
• small leucine-rich protein 1A

Background

Biglycan is encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BGN