BHMT Antibody (9E1)

Western Blot: BHMT Antibody (9E1) [NBP2-75418] - Analysis of BHMT on human liver tissue and human kidney tissue lysate using anti-BHMT antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: BHMT Antibody (9E1) [NBP2-75418] - Staining BHMT (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BHMT Antibody (9E1) [NBP2-75418] - Analysis of paraffin-embedded rat kidney tissue using anti-BHMT antibody. Counter stained with hematoxylin.

Flow Cytometry: BHMT Antibody (9E1) [NBP2-75418] - Analysis of HepG2 cells with BHMT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG1 was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: BHMT Antibody (9E1) [NBP2-75418] - Staining BHMT (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BHMT Antibody (9E1) [NBP2-75418] - Analysis of paraffin-embedded human liver tissue using anti-BHMT antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BHMT Antibody (9E1) [NBP2-75418] - Analysis of paraffin-embedded mouse liver tissue using anti-BHMT antibody. Counter stained with hematoxylin.

Product Discontinued

BHMT Antibody (9E1) Summary

• Immunogen: Recombinant protein with Human BHMT aa 250-406 / 406. (SwissProt: Q93088 Human; SwissProt: 329582 Mouse; SwissProt: O09171 Rat)
• Localization: Cytoplasm.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BHMT
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for BHMT Antibody (9E1)

• betaine homocysteine methyltransferase
• betaine-homocysteine methyltransferase
• betaine--homocysteine S-methyltransferase 1
• betaine--homocysteine S-methyltransferase
• BHMT1
• EC 2.1.1
• EC 2.1.1.5

Background

BHMT encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BHMT