beta Tubulin Antibody (SR25-04)

Western Blot: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Western blot analysis of beta Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat A

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Staining beta Tubulin in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-beta Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-4, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Analysis of NIH/3T3 cells with beta Tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Analysis of beta Tubulin on different cell lysates using anti-beta Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: PC12

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Staining beta Tubulin in PC12 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Staining beta Tubulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Staining beta Tubulin in SH-SY-5Y cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Staining beta Tubulin in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Analysis of paraffin-embedded human colon cancer tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Analysis of paraffin-embedded human kidney tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Analysis of paraffin-embedded mouse brain tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SR25-04) [NBP2-67341] - Analysis of paraffin-embedded mouse colon tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.

Product Discontinued

beta Tubulin Antibody (SR25-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human beta Tubulin aa 308-357 / 444. (SwissProt: P07437 Human; SwissProt: P99024 Mouse; SwissProt: P69897 Rat)
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TUBB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-400
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for beta Tubulin Antibody (SR25-04)

• beta polypeptide
• beta Tubulin
• MGC117247
• OK/SW-cl.56
• TUBB
• TUBB5
• tubulin beta chain
• tubulin beta polypeptide
• tubulin beta-1 chain
• Tubulin beta-5 chain
• tubulin, beta

Background

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Tubulin Antibody (NBP2-67341)(1)

Publication using NBP2-67341

• Cardani S, Janes T, Saini J et al. Etonogestrel Administration Reduces the Expression of PHOX2B and Its Target Genes in the Solitary Tract Nucleus International Journal of Molecular Sciences 2022-04-27 [PMID: 35563209] (WB, Rat)

Bioinformatics

• Gene Symbol: TUBB