Beta Dystroglycan Antibody (P38G1) - BSA Free

• Reactivity: Hu, Mu
• Applications: ELISA, Flow
• Clone: P38G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Beta Dystroglycan Antibody (P38G1) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The original antibody was generated by immunizing BALB/c mice with a mixture of transfected HEK 293 cells expressing human and mouse beta-DAG. (Uniprot# Q14118; Q62165)
• Specificity: This antibody recognizes human and mouse beta dystroglycan (beta-DAG). Beta-dystroglycan is a transmembrane protein that plays an important role in connecting the extracellular matrix to the cytoskeleton. It plays an important role in linking dystrophin to the laminin-binding alpha-dystroglycan. It acts as a cell adhesion receptor in both muscle and non-muscle tissues. It also functions in cell adhesion-mediated signaling and implicated in cell polarity.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Beta Dystroglycan Antibody (P38G1) - BSA Free

• A3a
• AGRNR
• DAG156DAG
• dystroglycan 1 (dystrophin-associated glycoprotein 1)
• dystroglycan
• Dystrophin-associated glycoprotein 1

Background

The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAG1