Beta Dystroglycan Antibody (GT9112) - Azide and BSA Free

Western Blot: Beta Dystroglycan Antibody (GT9112) [NBP3-13642] - Wild-type (WT) and Beta Dystroglycan knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Beta Dystroglycan antibody [GT9112] (NBP3-13642) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Beta Dystroglycan Antibody (GT9112) [NBP3-13642] - Beta Dystroglycan antibody [GT9112] detects Beta Dystroglycan protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse placenta. Beta Dystroglycan stained by Beta Dystroglycan antibody [GT9112] (NBP3-13642) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Beta Dystroglycan Antibody (GT9112) [NBP3-13642] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Beta Dystroglycan antibody [GT9112] (NBP3-13642) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: GT9112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Beta Dystroglycan Antibody (GT9112) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Beta Dystroglycan / beta Dystroglycan. The exact sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAG1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Beta Dystroglycan Antibody (GT9112) - Azide and BSA Free

• A3a
• AGRNR
• DAG156DAG
• dystroglycan 1 (dystrophin-associated glycoprotein 1)
• dystroglycan
• Dystrophin-associated glycoprotein 1

Background

The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAG1