Bcl-6 Overexpression Lysate (Native) (NBL1-07951)

Images

 
Western Blot: Bcl6 Overexpression Lysate (Adult Normal) [NBL1-07951] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bcl6.
Western Blot: Bcl6 Overexpression Lysate (Adult Normal) [NBL1-07951] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for BCL6.

Product Details

Summary
Applications WB

Order Details

Bcl-6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219007 and based on accession number NM_001706. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens B-cell CLL/lymphoma 6 (BCL6), transcript variant 1, mRNA.
Gene
BCL6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
78.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Bcl-6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Bcl-6 Overexpression Lysate (Native)

  • B-cell CLL/lymphoma 6
  • B-cell lymphoma 6 protein transcript
  • Bcl-5
  • BCL5B-cell lymphoma 5 protein
  • Bcl6
  • Bcl-6
  • BCL6A
  • cys-his2 zinc finger transcription factor
  • LAZ3
  • LAZ3ZBTB27B-cell lymphoma 6 protein
  • lymphoma-associated zinc finger gene on chromosome 3
  • Protein LAZ-3
  • ZBTB27
  • Zinc finger and BTB domain-containing protein 27
  • Zinc finger protein 51ZNF51
  • zinc finger transcription factor BCL6S
  • ZNF51

Background

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) and variant 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Bcl-6 Lysate (NBL1-07951) (0)

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Bioinformatics

Gene Symbol BCL6
Entrez