Bcl-2 related protein A1 Antibody

Product Discontinued

Bcl-2 related protein A1 Antibody Summary

• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Bfl-1. The immunogen is located within the first 50 amino acids of Bfl-1.
• Specificity: At least two isoforms of Bfl-1 are known to exist; this antibody will detect both isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-2 ug/ml
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Bcl-2 related protein A1 Antibody

• ACC-1
• ACC-2
• Bcl-2 related protein A1
• BCL2A1
• Bcl2L5
• Bcl2-L-5
• BCL2L5HBPA1
• Bcl-2-like protein 5
• BCL2-related protein A1
• Bfl-1
• BFL1bcl-2-related protein A1
• GRS
• GRSbcl2-L-5
• hematopoietic BCL2-related protein A1
• Hemopoietic-specific early response protein
• Protein BFL-1
• Protein GRS

Background

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL2A1
• Entrez:
  • 597(Human)
• Uniprot:
  • Q16548()