BCKDK Antibody

Product Discontinued

BCKDK Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human BCKD. The immunogen region is: N-terminal.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCKDK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BCKDK Antibody

• BCKDHKIN
• branched chain alpha-ketoacid dehydrogenase kinase
• branched chain ketoacid dehydrogenase kinase
• EC 2.7.11
• EC 2.7.11.4
• mitochondrial

Background

BCKDK belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. BCKDK catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. BCKDK is the key enzyme that regulates the activity state of the BCKD complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCKDK Antibody (NBP1-70814)(1)

Publication using NBP1-70814

• Lake AD, Novak P, Shipkova P et al. Branched chain amino acid metabolism profiles in progressive human nonalcoholic fatty liver disease. Amino Acids. 2014-12-23 [PMID: 25534430] (WB)

Bioinformatics

• Gene Symbol: BCKDK
• Entrez:
  • 10295(Human)
  • 12041(Mouse)
  • 29603(Rat)
• Uniprot:
  • O14874(Human)