BCKDHB Antibody (RB20925)

Western Blot: BCKDHB Antibody (RB20925) [NBP2-29984] - All lanes : Anti-BCKDHB Antibody (N-term). Lane 1: Hela whole cell lysate; Lane 2: 293T/17 whole cell lysate; Lane 3: Jurkat whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated. Predicted band size : 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunocytochemistry/Immunofluorescence: BCKDHB Antibody (RB20925) [NBP2-29984] - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling BCKDHB with NBP2-29984, followed by Dylight(R) 488-conjugated goat anti-Rabbit IgG secondary antibody (green). Immunofluorescence image showing Cytoplasm staining on Hela cell line. The nuclear counter stain is DAPI (blue).

Immunohistochemistry-Paraffin: BCKDHB Antibody (RB20925) [NBP2-29984] - Immunohistochemical analysis of paraffin-embedded Human kidney tissue using NBP2-29984 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Flow Cytometry: BCKDHB Antibody (RB20925) [NBP2-29984] - BCKDHB Antibody (N-term) (NBP2-29984) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot: BCKDHB Antibody (RB20925) [NBP2-29984] - All lanes : Anti-BCKDHB Antibody (N-term). Lane 1: Hela whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: Daudi whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated. Predicted band size : 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry-Paraffin: BCKDHB Antibody (RB20925) [NBP2-29984] - Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using NBP2-29984 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Product Discontinued

BCKDHB Antibody (RB20925) Summary

• Immunogen: This BCKDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human BCKDHB.
• Localization: Cellular Location Mitochondrion matrix.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCKDHB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:50
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Western Blot 1:1000
• Theoretical MW: 43.122 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.45 mg/ml
• Purity: Protein A purified

Alternate Names for BCKDHB Antibody (RB20925)

• 2-oxoisovalerate dehydrogenase beta subunit
• 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
• BCKDE1B
• BCKDH E1-beta
• branched chain alpha-ketoacid dehydrogenase E1-beta subunit
• branched chain keto acid dehydrogenase E1, beta polypeptide
• Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
• dJ279A18.1
• E1B
• E1b-beta subunit of the branched-chain complex
• EC 1.2.4.4
• FLJ17880

Background

Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria, and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit, and mutations therein have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation, and feeding problems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCKDHB
• Uniprot:
  • P21953(Human)