BCAP/PIK3AP1 Antibody [Unconjugated]

Images

 
Western Blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human/Mouse BCAP Antigen Affinity-purified ...read more
BCAP is hyperphosphorylated in B-cell, macrophages, and Expi293F cells.A, lysates from THP-1 and Ramos cells were dephosphorylated with lambda -phosphatase and immunoblotted for BCAP. B, His-Avi-tagged BCAP expressed ...read more
BCAP is phosphorylated by BTK, LYN, and SYK. Purified BCAP, dephosphorylated BCAP ( lambda -BCAP), and myelin basic protein (MyBP) were phosphorylated with (A) tyrosine kinases SYK, LYN, BTK, TYK2, and ITK, or (B) ...read more
BCAP is hyperphosphorylated in B-cell, macrophages, and Expi293F cells.A, lysates from THP-1 and Ramos cells were dephosphorylated with lambda -phosphatase and immunoblotted for BCAP. B, His-Avi-tagged BCAP expressed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

BCAP/PIK3AP1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human BCAP
Met462-Leu650
Accession # Q6ZUJ8
Specificity
Detects endogenous human and mouse BCAP in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIK3AP1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4857 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCAP/PIK3AP1 Antibody [Unconjugated]

  • 1810044J04Rik
  • B cell adaptor protein
  • BCAP
  • BCAPB-cell adapter for phosphoinositide 3-kinase
  • B-cell phosphoinositide 3-kinase adapter protein 1
  • FLJ35564
  • phosphoinositide 3-kinase adapter protein 1
  • phosphoinositide-3-kinase adaptor protein 1
  • Pik3ap1
  • RP11-34E5.3

Background

BCAP (B-cell adaptor for phosphoinositide 3-kinase (PI3K)) participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BCAP/PIK3AP1 Antibody (AF4857)(2)

We have publications tested in 2 confirmed species: Human, Escherichia coli.

We have publications tested in 2 applications: Bioassay, ICC.


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Bioassay
(1)
ICC
(1)
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Filter By Species
Human
(1)
Escherichia coli
(1)
All Species

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PIK3AP1
Uniprot