Western Blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Human/Mouse BCAP Antigen Affinity-purified ...read more
BCAP is hyperphosphorylated in B-cell, macrophages, and Expi293F cells.A, lysates from THP-1 and Ramos cells were dephosphorylated with lambda -phosphatase and immunoblotted for BCAP. B, His-Avi-tagged BCAP expressed ...read more
BCAP is phosphorylated by BTK, LYN, and SYK. Purified BCAP, dephosphorylated BCAP ( lambda -BCAP), and myelin basic protein (MyBP) were phosphorylated with (A) tyrosine kinases SYK, LYN, BTK, TYK2, and ITK, or (B) ...read more
BCAP is hyperphosphorylated in B-cell, macrophages, and Expi293F cells.A, lysates from THP-1 and Ramos cells were dephosphorylated with lambda -phosphatase and immunoblotted for BCAP. B, His-Avi-tagged BCAP expressed ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BCAP/PIK3AP1 Antibody [Unconjugated]
1810044J04Rik
B cell adaptor protein
BCAP
BCAPB-cell adapter for phosphoinositide 3-kinase
B-cell phosphoinositide 3-kinase adapter protein 1
FLJ35564
phosphoinositide 3-kinase adapter protein 1
phosphoinositide-3-kinase adaptor protein 1
Pik3ap1
RP11-34E5.3
Background
BCAP (B-cell adaptor for phosphoinositide 3-kinase (PI3K)) participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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