BCAP/PIK3AP1 Antibody (OTI7A11)

Western Blot: BCAP/PIK3AP1 Antibody (OTI7A11) [NBP2-03109] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3AP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3AP1.

Immunocytochemistry/Immunofluorescence: BCAP/PIK3AP1 Antibody (OTI7A11) [NBP2-03109] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PIK3AP1.

Immunohistochemistry-Paraffin: BCAP/PIK3AP1 Antibody (OTI7A11) [NBP2-03109] - Staining of paraffin-embedded Human lymphoma tissue using anti-PIK3AP1 mouse monoclonal antibody.

Flow Cytometry: BCAP/PIK3AP1 Antibody (OTI7A11) [NBP2-03109] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIK3AP1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: BCAP/PIK3AP1 Antibody (OTI7A11) [NBP2-03109] - Staining of paraffin-embedded Human Kidney tissue using anti-PIK3AP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAP/PIK3AP1 Antibody (OTI7A11) [NBP2-03109] - Staining of paraffin-embedded Human lymph node tissue using anti-PIK3AP1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI7A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.98 mg/ml

BCAP/PIK3AP1 Antibody (OTI7A11) Summary

• Description: Novus Biologicals Mouse BCAP/PIK3AP1 Antibody (OTI7A11) (NBP2-03109) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PIK3AP1 (NP_689552) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIK3AP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 90.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.98 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BCAP/PIK3AP1 Antibody (OTI7A11)

• 1810044J04Rik
• B cell adaptor protein
• BCAP
• BCAPB-cell adapter for phosphoinositide 3-kinase
• B-cell phosphoinositide 3-kinase adapter protein 1
• FLJ35564
• phosphoinositide 3-kinase adapter protein 1
• phosphoinositide-3-kinase adaptor protein 1
• Pik3ap1
• RP11-34E5.3

Background

PIK3AP1 is involved in the activation of phosphoinositide 3-kinase (PI3K) in B-cells and in natural killer (NK) cells.Couples B-cell antigen receptor (BCR) to PI3K activation by providing a docking site for the PI3K subunit PIK3R1,which contributes to B-cell develop

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIK3AP1
• Entrez:
  • 118788(Human)