BCAM/CD239 Antibody (BRIC221)

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Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
BRIC221
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

BCAM/CD239 Antibody (BRIC221) Summary

Additional Information
Clone BRIC221 was used by HLDA to establish CD designation.
Immunogen
Human erythrocytes
Specificity
NB100-66523 recognizes a non-polymorphic determinant on both the 85 and 78kD Lutheran (Lu) glycoproteins. BRIC 221 specifically recognizes an epitope in the fourth extracellular domain of Lu glycoprotein. Lutheran glycoprotein is a member of the immunoglobulin superfamily and has recently been designated CD239 (B-CAM) at the 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in the peripheral blood.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BCAM
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Non-reducing conditions required in Western Blotting.
Control
BCAM/CD239 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for BCAM/CD239 Antibody (BRIC221)

  • antigen identified by monoclonal F8
  • Auberger B antigen
  • basal cell adhesion molecule (Lu and Au blood groups)
  • basal cell adhesion molecule (Lutheran blood group)
  • basal cell adhesion molecule
  • B-CAM cell surface glycoprotein
  • BCAM
  • B-cell adhesion molecule
  • CD239 antigen
  • CD239
  • F8/G253 antigen
  • glycoprotein 95kDa
  • LUAU
  • Lutheran antigen
  • Lutheran blood group (Auberger b antigen included)
  • Lutheran blood group glycoprotein
  • MSK19

Background

CD239 glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. CD239 is expressed by erythrocytes in the peripheral blood and may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BCAM
Entrez
Uniprot