BAP1 Antibody (C.15200212)

Western Blot: BAP1 Antibody (C.15200212) [NBP2-59268] - IP was performed on 1 mg RIPA cell lysate from human NB4 cells (lane 1-3) or mouse M1 cells (lane 4-6) using the antibody against BAP1 diluted 1:400 (lane 3 and 6) or an IgG negative control (lane 2 and 5). The samples were analysed by Western blot analysis as described above. The input sample (30 ug RIPA lysate) was used as a positive control (lane 1 and 4).

Immunocytochemistry/Immunofluorescence: BAP1 Antibody (C.15200212) [NBP2-59268] - HeLa cells were stained with the antibody (left) diluted 1:1,000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Western Blot: BAP1 Antibody (C.15200212) [NBP2-59268] - Whole cell extracts from human NB4 cells (30 ug, lane 1) or mouse M1 cells (lane 2) were analyzed using the antibody against BAP1, diluted 1:1000. The position of the protein of interest (expected MW 80 kDa) is indicated on the right; the marker (in kDa) is shown on the left. Antibody titration on NB4 (1) and M1 (2) cells with the BAP1 antibody.

Product Discontinued

BAP1 Antibody (C.15200212) Summary

• Immunogen: The exact immunogen is proprietary information.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunoprecipitation 1:400
  • Western Blot 1:1000 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for BAP1 Antibody (C.15200212)

• BAP1
• BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
• BRCA1-associated protein 1
• Cerebral protein 6
• cerebral protein-13
• Cerebral Protein-6
• DKFZp686N04275
• EC 3.4.19.12
• FLJ35406
• FLJ37180
• hucep-6
• KIAA0272HUCEP-13
• ubiquitin carboxyl-terminal hydrolase BAP1
• ubiquitin carboxy-terminal hydrolase
• UCHL2

Background

BRCA1-associated protein 1 (BAP1) is a ubiquitin hydrolase that acts as a BRCA1 growth control pathway tumor suppressor. Specifically, BAP-1 localizes to the nucleus where it interferes with the BRCA1/BARD1 heterodimer activity and mediates deubiquitination of histone H2A and HCFC1. BAP 1 is highly expressed in testis, placenta, breast and ovary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAP1