BAIAP2 Antibody

Western Blot: BAIAP2 Antibody [NB100-827] - Staining of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: BAIAP2 Antibody [NB100-827] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BAIAP2 Antibody Summary

• Immunogen: Peptide with sequence C-SGSGTLVSTV corresponding to C-Terminus according to NP_059344.1.
• Specificity: This antibody is expected to recognise only human isoform 1 according to NP_059344.1.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BAIAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human brain lysates (calculated MW of 57.4 kDa band according to NP_059344.1). IHC: Paraffin embedded Human Brain (Cortex).
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  BAIAP2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BAIAP2 Antibody

• BAI1-associated protein 2IRS-58
• BAI-associated protein 2
• BAP2
• brain-specific angiogenesis inhibitor 1-associated protein 2
• Fas ligand-associated factor 3
• FLAF3
• Insulin receptor substrate p53
• Insulin receptor substrate p53/p58
• Insulin receptor substrate protein of 53 kDa
• IRSP53
• IRSp53/58
• Protein BAP2

Background

BAIAP2, a target of p53, has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This interaction at the cytoplasmic membrane is crucial to the function of this protein, which may be involved in neuronal growth-cone guidance. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This antibody is expected to recognise the human isoform 1 only of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAIAP2
• Entrez:
  • 10458(Human)
  • 108100(Mouse)
  • 117542(Rat)
• Uniprot:
  • NP_059344.(Human)