BAFF/BLyS/TNFSF13B Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC204672 and based on accession number NM_006573. The protein contains a C-terminal DDK tag.
Homo sapiens tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B), transcript variant 1, mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Lysate Details for BAFF/BLyS/TNFSF13B
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for BAFF/BLyS/TNFSF13B Overexpression Lysate (Native)
- ApoL related ligand TALL-1
- B lymphocyte stimulator
- BAFFB-cell activating factor
- B-cell-activating factor
- BLYSB-lymphocyte stimulator
- CD257 antigen
- Dendritic cell-derived TNF-like molecule
- TALL-1delta BAFF
- TALL1Delta4 BAFF
- TNF- and APOL-related leukocyte expressed ligand 1
- TNF and ApoL-related leukocyte expressed ligand 1
- TNF homolog that activates apoptosis
- tumor necrosis factor (ligand) superfamily, member 13b
- tumor necrosis factor (ligand) superfamily, member 20
- tumor necrosis factor ligand superfamily member 13B
- tumor necrosis factor superfamily, member 13b
- tumor necrosis factor-like protein ZTNF4
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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