BAFF/BLyS/TNFSF13B Overexpression Lysate (Native)

Images

 
Western Blot: TNFSF13B Overexpression Lysate (Adult Normal) [NBL1-17166] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF13B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BAFF/BLyS/TNFSF13B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204672 and based on accession number NM_006573. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B), transcript variant 1, mRNA.
Gene
TNFSF13B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for BAFF/BLyS/TNFSF13B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BAFF/BLyS/TNFSF13B Overexpression Lysate (Native)

  • ApoL related ligand TALL-1
  • B lymphocyte stimulator
  • BAFF
  • BAFFB-cell activating factor
  • B-cell-activating factor
  • BLyS
  • BLYSB-lymphocyte stimulator
  • CD257 antigen
  • CD257
  • Dendritic cell-derived TNF-like molecule
  • DTL
  • TALL1
  • TALL-1delta BAFF
  • TALL1Delta4 BAFF
  • THANK
  • TNF- and APOL-related leukocyte expressed ligand 1
  • TNF and ApoL-related leukocyte expressed ligand 1
  • TNF homolog that activates apoptosis
  • TNFSF13B
  • TNFSF20
  • tumor necrosis factor (ligand) superfamily, member 13b
  • tumor necrosis factor (ligand) superfamily, member 20
  • tumor necrosis factor ligand superfamily member 13B
  • tumor necrosis factor superfamily, member 13b
  • tumor necrosis factor-like protein ZTNF4
  • ZTNF4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-97587
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-56516
Species: Hu, Mu
Applications: WB, IHC
AF1162
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
NBP1-97637
Species: Hu
Applications: WB, B/N, ELISA, Flow, ICC/IF, IHC
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
NBP2-37585
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC
AF3589
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NBP1-19097
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
NBP2-25196
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, In vitro, CyTOF-ready
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00054474-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166) (0)

There are no publications for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166) (0)

There are no reviews for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional BAFF/BLyS/TNFSF13B Products

Bioinformatics Tool for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166)

Discover related pathways, diseases and genes to BAFF/BLyS/TNFSF13B Lysate (NBL1-17166). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166)

Discover more about diseases related to BAFF/BLyS/TNFSF13B Lysate (NBL1-17166).
 

Pathways for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166)

View related products by pathway.

PTMs for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166)

Learn more about PTMs related to BAFF/BLyS/TNFSF13B Lysate (NBL1-17166).
 

Research Areas for BAFF/BLyS/TNFSF13B Lysate (NBL1-17166)

Find related products by research area.

Blogs on BAFF/BLyS/TNFSF13B

There are no specific blogs for BAFF/BLyS/TNFSF13B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BAFF/BLyS/TNFSF13B Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFSF13B
Entrez