BAFF/BLyS/TNFSF13B Antibody Pair [Biotin]

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin
• Suitable Sample Type: Cell Culture Supernatant, Plasma, Serum
• Standard Curve Range: 16 - 1000 pg/ml
• Sensitivity: 16 pg/ml

BAFF/BLyS/TNFSF13B Antibody Pair [Biotin] Summary

• Description: Sandwich ELISA for the quantitative determination of Human BAFF/BLyS/TNFSF13B
• Specificity: Detects human BAFF/BLyS/TNFSF13B. Does not detect mouse BAFF/BLyS/TNFSF13B.
• Standard Curve Range: 16 - 1000 pg/ml
• Sensitivity: 16 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: TNFSF13B

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA
• Application Notes: 1. Dilute the desired amount of Coating Antibody (COAT) (1 mg/ml) (rat monoclonal antibody) to 2ug/ml in PBS without carrier protein and use it fresh.
  2. Dilute the desired amount of Detection Antibody (DET) (1 mg/ml) (biotinylated rat monoclonal antibody) to 1 ug/ml in Blocking Buffer and use it fresh.
  3. Reconstitute the Streptavidin-HRP (STREP) with 100 ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted STREP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 10 ul in 10 ml of ELISA Buffer (1:1000).
  4. Reconstitute the Standard Protein (STD) (recombinant human BAFF/BLyS/TNFSF13B) with 100 ul ELISA Buffer to obtain a concentration of 50 ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/ thaw cycles. A standard curve using 2-fold serial dilutions in ELISA Buffer is recommended. Suggested standard points are 1000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.25 pg/ml, 15.6 pg/ml and 0 pg/ml.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for BAFF/BLyS/TNFSF13B Antibody Pair [Biotin]

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

TNFSF13B recognizes membrane bound and soluble human and mouse(weak) BAFF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF13B