BAFF/BLyS/TNFSF13B Antibody (121834) [Unconjugated]

BAFF/BLyS/TNFSF13B was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse BAFF/BLyS/TNFSF13B Monoclonal Antibody (Catalog # MAB13571) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: ICC/IF
• Clone: 121834
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

BAFF/BLyS/TNFSF13B Antibody (121834) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse BAFF/BLyS/TNFSF13B
  Ala127-Leu309
  Accession # Q9WU72
• Specificity: Detects mouse BAFF/BLyS/TNFSF13B in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFF/BLyS/TNFSF13B Antibody (121834) [Unconjugated]

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

BAFF (also known as TALL-1, BLyS, THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 aa protein consisting of a 248 aa extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor(BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6 - 8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is upregulated by IFN-alpha , IFN-beta , LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145. 
4. Liu, Y. et al. (2002) Cell 108:383. 
5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313. 
6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
7. Thompson, J.S. et al. (2001) Science 293:2108.
8. Ng, L. G. et al. (2004) J. Immunol. 173:807.
9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9WU72()