BAF53A Antibody (JU40-32)

Western Blot: BAF53A Antibody (JU40-32) [NBP2-75415] - Analysis of ACTL6A on Jurkat cell(1) and mouse brain tissue(2) lysate using anti-ACTL6A antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: BAF53A Antibody (JU40-32) [NBP2-75415] - Staining ACTL6A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BAF53A Antibody (JU40-32) [NBP2-75415] - Analysis of paraffin-embedded mouse brain tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: BAF53A Antibody (JU40-32) [NBP2-75415] - Staining ACTL6A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BAF53A Antibody (JU40-32) [NBP2-75415] - Analysis of paraffin-embedded human tonsil tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BAF53A Antibody (JU40-32) [NBP2-75415] - Analysis of paraffin-embedded human spleen tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.

Product Discontinued

BAF53A Antibody (JU40-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human BAF53A aa 185-326 . (SwissProt: O96019 Human; SwissProt: Q9Z2N8 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACTL6A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BAF53A Antibody (JU40-32)

• actin-like 6A
• actin-like protein 6A
• actin-related protein 4,53 kDa BRG1-associated factor A
• Actin-related protein Baf53a
• Actl6
• Arp4
• arpNbeta
• BAF complex 53 kDa subunit
• Baf53a
• BAF53AACTL6
• BAF53MGC5382
• BRG1-associated factor 53A
• hArpN beta
• INO80 complex subunit K
• INO80KARPN-BETA

Background

BAF53A encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTL6A