Axin-1 Antibody (PSH03-00)

Western Blot: Axin-1 Antibody (PSH03-00) [NBP3-32064] - Western blot analysis of Axin-1 on different lysates with Rabbit anti-Axin-1 antibody (NBP3-32064) at 1/2,000 dilution. Lane 1: SW480 cell lysate (10 ug/Lane) Lane 2: HeLa cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: K-562 cell lysate (10 ug/Lane) Lane 5: Huh7 cell lysate (10 ug/Lane) Predicted band size: 95.6 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32064) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Axin-1 Antibody (PSH03-00) [NBP3-32064] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin-1 antibody (NBP3-32064) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32064) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: PSH03-00
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Axin-1 Antibody (PSH03-00) Summary

• Description: Novus Biologicals Rabbit Axin-1 Antibody (PSH03-00) (NBP3-32064) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human Axin-1 aa 1-400 / 862. (Uniprot: O15169)
• Localization: Cytoplasm. Nucleus. Membrane. Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AXIN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:2000
• Theoretical MW: 95.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Axin-1 Antibody (PSH03-00)

• axin 1
• Axin1
• Axin-1
• AXINaxis inhibitor 1
• Axis inhibition protein 1
• fused, mouse, homolog of
• hAxin
• MGC52315

Background

Negative regulator of Wnt signaling. Also associated with cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AXIN1