Axin-1 Antibody (4E9F1) - BSA Free

Western Blot: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis using AXIN1 mAb against human AXIN1 (AA: 546-752) recombinant protein. (Expected MW is 48.7 kDa)

Immunocytochemistry/Immunofluorescence: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis of Hela cells using AXIN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Flow Cytometry: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis of Hela cells using AXIN1 mouse mAb (green) and negative control (red).

Western Blot: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis using AXIN1 mAb against HEK293 (1) and AXIN1 (AA: 546-752)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Axin-1 Antibody (4E9F1) [NBP2-61695] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 4E9F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Axin-1 Antibody (4E9F1) - BSA Free Summary

• Description: Novus Biologicals Mouse Axin-1 Antibody (4E9F1) - BSA Free (NBP2-61695) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Axin-1 (AA: 546-752) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AXIN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Western Blot 1:500-1:2000
• Theoretical MW: 95.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Axin-1 Antibody (4E9F1) - BSA Free

• axin 1
• Axin1
• Axin-1
• AXINaxis inhibitor 1
• Axis inhibition protein 1
• fused, mouse, homolog of
• hAxin
• MGC52315

Background

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axin-1 Antibody (NBP2-61695)(1)

Publication using NBP2-61695

• KarLee M Understanding Vascular Calcification Through the Lens of Canonical WNT Signaling Thesis 2023-01-01 (Immunocytochemistry/ Immunofluorescence, Human)

Review for Axin-1 Antibody (NBP2-61695) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Immunofluorescence of cultured human cells Human 08/01/2025

Summary

• Application: Other
• Sample Tested: HEK 293 cell line,HEK 293,HEK293 cells
• Species: Human
• Lot: check order

Comments

• Comments: This antibody DOES NOT WORK. It only gives a background haze when used for immunofluorescence. DO NOT waste your money purchasing this antibody or anything else from this company. The images provided by them are not real and do not show the real performance of the product. Thank goodness we only wasted money in the trial size. The only reason there are no more bad reviews is that they make it impossible to post a review or complain.

Bioinformatics

• Gene Symbol: AXIN1