Mouse Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric)

ELISA: Mouse Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric) [NBP2-66733] - Samples were spiked with high concentrations of Mouse Atrial Natriuretic Peptide/ANP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric) [NBP2-66733] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Mouse Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse ANP in samples. No significant cross-reactivity or interference between Mouse ANP and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 4.65%
• Intra-Assay: CV% < 5.04%
• Spike Recovery: 84-109%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NPPA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric)

• ANF
• ANP
• ANPatriopeptin
• ATFB6
• atrial natriuretic factor
• Atrial Natriuretic Peptide
• Cardionatrin
• CDD-ANF
• natriuretic peptide A
• natriuretic peptide precursor A
• NPPA
• PND
• PNDcardionatrin
• prepronatriodilatin

Background

Atrial natriuretic factor (ANP) is a potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis and has a cGMP stimulating activity. There are two different prepronatriodilatin alleles. One codes for 2 Arginine residues at the C terminus that are cleaved to form the mature peptide, while the other ends in a termination codon immediately after the last codon of the mature peptide. ANP belongs to the natriuretic peptide family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Atrial Natriuretic Peptide/ANP ELISA Kit (NBP2-66733)(1)

Publication using NBP2-66733

• Kang JO, Ha TW, Jung HU et al. A cardiac-null mutation of Prdm16 causes hypotension in mice with cardiac hypertrophy via increased nitric oxide synthase 1 PloS one 2022-07-21 [PMID: 35862303] (ELISA)

FAQs for Atrial Natriuretic Peptide/ANP ELISA Kit (NBP2-66733). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NPPA