Rat Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Nppa (Rat) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of rat Nppa. |
| Standard Curve Range |
0.25 to 4 ng/mL |
| Sensitivity |
0.2 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 7.10% |
| Intra-Assay |
CV% < 4.80% |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
NPPA |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
| Publications |
|
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Rat ANP antibody (70x): A 70-fold biotinylated polyclonal antibody against rat ANP.
- Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine
- MIX Diluent Concentrate (10x): A 10-fold buffered protein base
- Rat ANP Microplate: A 96 well polystyrene microplate (12 strips of 8 wells) coated with apolyclonal antibody against rat ANP
- Rat ANP Standard: Rat ANP in a buffered protein base, lyophilized.
- Sealing Tapes: pressure-sensitive sealing tapes that can be cut to fit the format of theindividual assay.
- Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction
- Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Rat Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric)
Background
Atrial natriuretic factor (ANP) is a potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis and has a cGMP stimulating activity. There are two different prepronatriodilatin alleles. One codes for 2 Arginine residues at the C terminus that are cleaved to form the mature peptide, while the other ends in a termination codon immediately after the last codon of the mature peptide. ANP belongs to the natriuretic peptide family.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for Atrial Natriuretic Peptide/ANP ELISA Kit (KA0503)(3)
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Product General Protocols
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FAQs for Atrial Natriuretic Peptide/ANP ELISA Kit (KA0503). (Showing 1 - 1 of 1 FAQs).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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