Recombinant Human ATPG His Protein

SDS-Page: ATPG Protein [NBP2-22699]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human ATPG His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 26-298 of Human ATPG
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSATLKDIT RRLKSIKNIQ KITKSMKMVA AAKYARAERE LKPARIYGLG SLALYEKADI KGPEDKKKHL LIGVSSDRGL CGAIHSSIAK QMKSEVATLT AAGKEVMLVG IGDKIRGILY RTHSDQFLVA FKEVGRKPPT FGDASVIALE LLNSGYEFDE GSIIFNKFRS VISYKTEEKP IFSLNTVASA DSMSIYDDID ADVLQNYQEY NLANIIYYSL KESTTSEQSA RMTAMDNASK NASEMIDKLT LTFNRTRQAV ITKELIEIIS GAAALD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ATP5F1C
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 32.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human ATPG His Protein

• ATP synthase gamma chain, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• ATP5CATP synthase subunit gamma, mitochondrial
• ATP5CL1mitochondrial ATP synthase, gamma subunit 1
• F-ATPase gamma subunit

Background

ATP synthase subunit gamma, mitochondrial isoform L (liver), also known as ATP5C1, catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. Recombinant human ATP5C1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ATP5F1C