ATPase Colorimetric Assay Kit

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Product Details

Summary
Kit Type
Colorimetric Assay Kit

Order Details

ATPase Colorimetric Assay Kit Summary

Description
This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock kit and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock, a non-radioactive, superior phosphate detection reagent.

Key Features of PiColorLock:
  • Colorimetric Assay-competitor assays are radioactive.
  • Special additive ensures low backgrounds with acid-labile substrates.
  • Unparalleled stability of phosphate-dye complexes.
  • Reagent is compatible with almost any assay buffer.
  • No inhibition of color development by high concentrations of protein.
  • Stable reagent formulation - long shelf life.
Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.
Kit Type
Colorimetric Assay Kit
Gene
DNAH8

Applications/Dilutions

Application Notes
In vitro assay as reported in the literature (PMID: 21304972)
Publications
Read Publications using 601-0121.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 x 1.5 ml of 0.1M MgCl2 (2 x 1.5 ml)
  2. 1 x 10 ml of PiColorLock (1 x 25 ml)
  3. 1 x 0.25 ml of Accelerator (1 x 0.5 ml)
  4. 1 x 5 ml of 0.5M Tris pH 7.5 (1 x 10 ml)
  5. 1 x 5 ml of 0.1mM Pi standard (1 x 10 ml)
  6. 1 x 5 ml of Stabiliser (1 x 10 ml)
  7. 2 x 96-well plates (5 plates)
  8. 4 vials of lyophilized ATP (10 vials)

Notes

This prodcut is manufacture by Innova Biosciences.

Alternate Names for ATPase Colorimetric Assay Kit

  • ATPase
  • axonemal
  • dynein, axonemal, heavy chain 8
  • dynein, axonemal, heavy polypeptide 8
  • FLJ25850
  • FLJ36115
  • FLJ36334
  • hdhc9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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601-0121
Applications: In vitro

Publications for ATPase Kit (601-0121)(45)


Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using 601-0121 Applications Species
Sarraf NS, Shi R, McDonald L et al. Suramin Inhibits Hsp104 ATPase and Disaggregase Activity. PLoS One 2014 [PMID: 24945826]
Xiao X, Yang J, Li R et al. A helminth cathelicidin-like protein suppresses antigen processing and presentation in macrophages via inhibition of lysosomal vATPase. FASEB J. 2014 [PMID: 24391795]
Matos M, Augusto E, Agostinho P et al. Antagonistic Interaction between Adenosine A2A Receptors and Na+/K+-ATPase-alpha 2 Controlling Glutamate Uptake in Astrocytes. J Neurosci 2013 [PMID: 24259572]
Luo H, Xu HQ, Chen X et al. Potent in vitro Interference of Fleroxacin in DNA-binding, Unwinding and ATPase Activities of Bloom Helicase. Biomed Environ Sci. 2013 [PMID: 23534463]
Bruchmann A, Roller C, Walther TV et al. Bcl-2 associated athanogene 5 Bag5is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis. BMC Cancer 2013 [PMID: 23448667]
Ronchi D, Di Fonzo A, Lin W et al. Mutations in DNA2 Link Progressive Myopathy to Mitochondrial DNA Instability. Am J Hum Genet. 2013 [PMID: 23352259]
Choi CH, Spooner R, DeGuzman J et al. P. gingivalis-Nucleoside-diphosphate-kinase Inhibits ATP-Induced Reactive-Oxygen-Species via P2X7 Receptor / NADPH-Oxidase Signaling and Contributes to Persistence. Cell Microbiol 2012 [PMID: 23241000]
Robinson MW, Alvarado R, To J et al. Physiological and biochemical characteristics of laboratory induced mutants of Botrytis cinerea with resistance to fluazinam. FASEB J. 2014 [PMID: 22872675]
Baker N, Zhang G, You Y, Tuan R. Caveolin-1 regulates proliferation and osteogenic differentiation of human mesenchymal stem cells. J Cell Biochem 2012 [PMID: 22807396]
Podhajska A, Musso A, Trancikova A et al. Common Pathogenic Effects of Missense Mutations in the P-Type ATPase ATP13A2 PARK9 Associated with Early-Onset Parkinsonism. PLoS One 2012 [PMID: 22768177]
Show All 45 Publications.

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FAQs for ATPase Kit (601-0121). (Showing 1 - 4 of 4 FAQs).

  1. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.

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Bioinformatics

Gene Symbol DNAH8