ATPase Colorimetric Assay Kit

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Product Details

Summary
Kit Type
Colorimetric Assay Kit

Order Details

ATPase Colorimetric Assay Kit Summary

Description
This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock kit and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock, a non-radioactive, superior phosphate detection reagent.

Key Features of PiColorLock:
  • Colorimetric Assay-competitor assays are radioactive.
  • Special additive ensures low backgrounds with acid-labile substrates.
  • Unparalleled stability of phosphate-dye complexes.
  • Reagent is compatible with almost any assay buffer.
  • No inhibition of color development by high concentrations of protein.
  • Stable reagent formulation - long shelf life.
Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.
Kit Type
Colorimetric Assay Kit
Gene
DNAH8

Applications/Dilutions

Application Notes
In vitro assay as reported in the literature (PMID: 21304972)
Publications
Read Publications using 601-0121.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 x 0.25 ml of Accelerator (1 x 0.5 ml)
  2. 1 x 1.5 ml of 0.1M MgCl2 (2 x 1.5 ml)
  3. 1 x 10 ml of PiColorLock (1 x 25 ml)
  4. 1 x 5 ml of Stabiliser (1 x 10 ml)
  5. 1 x 5 ml of 0.1mM Pi standard (1 x 10 ml)
  6. 1 x 5 ml of 0.5M Tris pH 7.5 (1 x 10 ml)
  7. 2 x 96-well plates (5 plates)
  8. 4 vials of lyophilized ATP (10 vials)

Notes

This prodcut is manufacture by Innova Biosciences.

Alternate Names for ATPase Colorimetric Assay Kit

  • ATPase
  • axonemal
  • dynein, axonemal, heavy chain 8
  • dynein, axonemal, heavy polypeptide 8
  • FLJ25850
  • FLJ36115
  • FLJ36334
  • hdhc9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Publications for ATPase Kit (601-0121)(70)


Showing Publications 1 - 10 of 70. Show All 70 Publications.
Publications using 601-0121 Applications Species
Zadorozhny K, Sannino V, Belan O et al. Fanconi-Anemia-Associated Mutations Destabilize RAD51 Filaments and Impair Replication Fork Protection. Cell Rep. 2017 Oct 10 [PMID: 29020621]
Granja AG, Tafalla C. Different IgM+ B cell subpopulations residing within the peritoneal cavity of vaccinated rainbow trout are differently regulated by BAFF. Fish Shellfish Immunol. 2017 Oct 5 [PMID: 28989090]
Park SA, Hong BZ, Ha KC et al. Protein tyrosine phosphatase 1B is a mediator of cyclic ADP ribose-induced Ca2+ signaling in ventricular myocytes. Exp Mol Med. 2017 Jun 2 [PMID: 28572573]
Bittencourt IA, Serpeloni M, Hiraiwa PM et al. Dissecting biochemical peculiarities of the ATPase activity of TcSub2, a component of the mRNA export pathway in Trypanosoma cruzi. Int J Biol Macromol. 2017 [PMID: 28212935]
Huerta-Uribe A, Marjenberg ZR, Yamaguchi N et al. Identification and Characterization of Novel Compounds Blocking Shiga Toxin Expression in Escherichia coli O157:H7. Front Microbiol. 2016 Nov 30 [PMID: 27965652]
Martino L, Holland L, Christodoulou E et al. The Biophysical Characterisation and SAXS Analysis of Human NLRP1 Uncover a New Level of Complexity of NLR Proteins. PLoS One. 2016 Oct 11 [PMID: 27727326]
Sheng Y, Yang X, Lian Y et al. Characterization of a cadmium resistance Lactococcus lactissubsp. lactis strain by antioxidant assays and proteome profiles methods. Environ Toxicol Pharmacol. 2016 [PMID: 27522548]
Kristensen ML, Kierulf-Lassen C, Nielsen PM et al. Remote ischemic perconditioning attenuates ischemia/reperfusion-induced downregulation of AQP2 in rat kidney. Physiol Rep. 2016 [PMID: 27405971]
Wambach JA, Yang P, Wegner DJ et al. Functional Characterization of ABCA3 Mutations from Infants with Respiratory Distress Syndrome. Am J Respir Cell Mol Biol. 2016 [PMID: 27374344]
Wang Y, Sun Y, Zhang Y et al. Antifungal Activity and Biochemical Response of Cuminic Acid against Phytophthora capsici Leonian. Molecules. 2016 Jun 11 [PMID: 27294911]
Show All 70 Publications.

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FAQs for ATPase Kit (601-0121). (Showing 1 - 5 of 5 FAQs).

  1. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.
  5. What is the ratio between ATP and ATPase in terms of molar, which may be appropriate for this assay?
    • There is no real definitive answer to this, as it depends on a variety of different things. The simple answer however is that there needs to be a huge excess of substrate. There needs to be enough substrate so this does not run out over the course of the assay, and stays in excess even as the enzyme reacts here, and increases the concentration of products.

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Bioinformatics

Gene Symbol DNAH8