ATPase Antibody

Product Discontinued

ATPase Antibody Summary

• Immunogen: A synthetic peptide (C-ELHRQEEAKQVLRYY) as part of mouse ATP13A2 protein (aa: 147-161) conjugated to KLH
• Localization: Accession Number: ATPase_MOUSE
• Specificity: Immunohistochemistry shows specific staining for ATPase.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNAH8
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Reactivity Notes

Reacts with rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized water. Centrifuge to remove any insoluble material.

Alternate Names for ATPase Antibody

• ATPase
• axonemal
• dynein, axonemal, heavy chain 8
• dynein, axonemal, heavy polypeptide 8
• FLJ25850
• FLJ36115
• FLJ36334
• hdhc9

Background

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATPase Antibody (R-073-100)(14)

Publications using R-073-100

• Harauz G, Ishiyama N, Hill CM, Bates IR, Libich DS, Fares C. Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis. . Micron. 35(7):503-42. 2004-01-01 [PMID: 15219899]
• Young JM, Shykind BM, Lane RP, Tonnes-Priddy L, Ross JA, Walker M, Williams EM, Trask BJ. Odorant receptor expressed sequence tags demonstrate olfactory expression of over 400 genes, extensive alternate splicing and unequal expression levels. Genome Biol. 4(11):R71. 2003-01-01 [PMID: 14611657]
• Schwartz et al. Prog Brain Res 137, 401-6. 2002-01-01 [PMID: 12440382]
• Hauben et al. J Clin Invest 108, 591-9. [PMID: 11518733]
• Yoleset al. J Neurosci 213740-8. 2001-06-01 [PMID: 11356861]
• Haubenet al. J Neurosci 206421-30. 2000-09-01 [PMID: 10964948]
• Givogriet al. J Neurosci Res 59153-9. 2000-01-15 [PMID: 10650873]
• Kim et al. Int J Biochem Cell Biol 29, 743-51. [PMID: 9251242]
• Matsuo, A et al. Am. J. Pathol. 150(4): 1253-1266. 1997-01-01 [PMID: 9094982]
• Kalwy SA, Smith R. Mechanisms of myelin basic protein and proteolipid protein targeting in oligodendrocytes (review). Mol Membr Biol 1167-78. [PMID: 7522797]
• Brophy et al. Trends Neurosci 16, 515-21. [PMID: 7509522]
• Wajgt et al. Acta Neurol Sc 68, 337-43. [PMID: 6198864]
• Day ED, Potter NT. Monoclonal and polyclonal antibodies to myelin basic protein determinants. J Neuroimmunol 10, 289-312. [PMID: 2418054]
• Mikoshiba K, Aruga J, Okano H. Molecular biology of myelin basic protein: gene rearrangement and expression of anti-sense RNA in myelin-deficient mutants. Comp Biochem Physiol C 98, 51-61. 1991-01-01 [PMID: 1709079]

FAQs for ATPase Antibody (R-073-100). (Showing 1 - 4 of 4 FAQs).

1. I have phosphate in my enzyme. What can I do?
   • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
   • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
   • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
   • Yes, only the phosphate will give a signal; pyrophosphate will not.

Bioinformatics

• Gene Symbol: DNAH8
• Entrez:
  • 1769(Human)