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ATP13A2 Blocking Peptide (NB110-41486PEP)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Concentration
1.0 mg/ml

Order Details

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ATP13A2 Blocking Peptide Summary

Description
A peptide to ATP13A2.
Protein/Peptide Type
Blocking Peptide
Gene
ATP13A2

Applications/Dilutions

Application Notes
This peptide is useful as a blocking peptide for NB110-41486.
IHC and WB were reported in scientific literature.

For further blocking peptide related protocol, click here.
Publications
Read Publication using
NB110-41486PEP in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Peptide dissolved in dH2O. Contains no BSA.
Preservative
No Preservative
Concentration
1.0 mg/ml

Alternate Names for ATP13A2 Blocking Peptide

  • ATPase type 13A2
  • EC 3.6.3
  • EC 3.6.3.-
  • EC 3.6.3.5
  • EC 3.6.3.8
  • FLJ26510
  • HSA9947
  • KRPPD
  • PARK9putative ATPase
  • Parkinson disease (autosomal recessive) 9 (Kufor-Rakeb syndrome)
  • probable cation-transporting ATPase 13A2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB300-270] - Park7(DJ-1) detected in human cortex.
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Immunohistochemistry-Paraffin: alpha-Synuclein [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using alpha-Synuclein antibody (clone 24.8) at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic puncta and varicosities staining was observed. Staining was performed by Histowiz.
Flow Cytometry: Synuclein-alpha [Nitrate Tyr125, Nitrate Tyr133] Antibody (24.8) [NBP1-26380] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using Synuclein-alpha [Nitrate Tyr125, Nitrate Tyr133] antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Western Blot: PINK1 Antibody [BC100-494] - Analysis of PINK1 in mouse liver and hypatocytes using PINK1 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - Immunocytochemistry of PINK1 antibody (BC100-494 Lot G). HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP.
Western Blot: PINK1 Antibody [BC100-494] - Analysis of PINK1 in HeLa whole cell lysate with and without treatment of 10 uM CCCP. Image courtesy of an anonymous customer review.
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Immunohistochemistry: SUG1 Antibody [NB100-2572] - Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti- TRIP1(SUG1) used at a dilution of 1:200 (1ug/ml). Detection: DAB
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Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti LAMP-2 (H4B4) [NBP2-22217] at a 1:100 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Antibody [Unconjugate
AF5528
Western blot shows lysates of human brain cortex, Alzheimer grey matter and white matter, and human cerebellum, brain cortex, and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Human beta -Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5528) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for beta -Synuclein  at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
beta -Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (globus pallidus) using Sheep Anti-Human beta -Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5528) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the presynaptic terminals. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: WB, IHC
SPG11 Antibody
NBP1-82143
Immunocytochemistry/Immunofluorescence: SPG11 Antibody [NBP1-82143] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: SPG11 Antibody [NBP1-82143] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for ATP13A2 Peptide (NB110-41486PEP)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, WB.


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Additional ATP13A2 Products

Related Products by Gene

FBXO7, GBA, GLUCOCEREBROSIDASE, HTRA2, KARS, LAMP2, LRRK2, MAPT, PARK2, PARK7, PARKIN PROTEIN, PINK1, PLA2G6, PSMC5, SLC6A3, SNCA, SNCB, SPG11, UCHL1, VCP

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Simple Western: ATP13A2 Antibody [NB110-41486] - Simple Western lane view shows a specific band for ATP13A2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB300-268] - Mouse CAD cells transfected with Human wild-type LRRK-2 (1:2,000).
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Immunohistochemistry: LRRK2 Antibody [NB300-268] - Brain, Putamen, Neurons and Glia 60x.
LRRK2 Antibody
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Bioinformatics

Gene Symbol ATP13A2