ATP11A Antibody

Immunocytochemistry/Immunofluorescence: ATP11A Antibody [NBP1-88903] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.

Immunohistochemistry-Paraffin: ATP11A Antibody [NBP1-88903] - Staining of human kidney shows moderate to strong positivity in apical membrane in cells in tubules.

Immunohistochemistry-Paraffin: ATP11A Antibody [NBP1-88903] - Staining of human endometrium shows moderate positivity in apical membrane in glandular cells.

Immunohistochemistry-Paraffin: ATP11A Antibody [NBP1-88903] - Staining of human testis shows weak to moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: ATP11A Antibody [NBP1-88903] - Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ATP11A Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SATGEIYLFCKGADSSIFPRVIEGKVDQIRARVERNAVEGLRTLCVAYKRLIQEEYEGICKLLQAAKVALQDREKKLAEAYEQIEKDLTLLG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP11A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ATP11A Protein (NBP1-88903PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ATP11A Antibody

• ATPase, class VI, type 11A

Background

The protein encoded by the ATP11A gene is an integral membrane ATPase. The encoded protein is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium across membranes. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP11A