ATP synthase C mature Overexpression Lysate

Images

 
Western Blot: ATP synthase C mature Overexpression Lysate (Adult Normal) [NBL1-07823] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATP synthase C mature.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ATP synthase C mature Overexpression Lysate Summary

Description
Transient overexpression lysate of ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9) (ATP5G1), nuclear gene encoding mitochondrial protein, transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC216197 and based on accession number NM_001002027. The protein contains a C-MYC/DDK Tag.
Gene
ATP5MC1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ATP synthase C mature Overexpression Lysate

  • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9)
  • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9)
  • ATP5A
  • ATP5G
  • ATPase protein 9
  • ATPase subunit 9
  • H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9)
  • isoform 1
  • mitochondrial ATP synthase, subunit 9
  • mitochondrial ATP synthase, subunit C
  • mitochondrial

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00000514-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-43782
Species: Hu
Applications: WB
NB300-221
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
H00000518-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
NB600-533
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IP, Simple Western, WB
NBP1-32870
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
NBP1-87408
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-97838
Species: Hu
Applications: IHC, IHC-P, IP, WB
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
AF1029
Species: Mu
Applications: ICC, IHC, IP, WB
NBP2-03417
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP1-84847
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB300-537
Species: Bv, Hu, Mu, Rb, Rt
Applications: ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
NBP1-90824
Species: Hu
Applications: ICC/IF, WB
NBP2-92677
Species: Hu, Mu, Rt
Applications: WB
NBL1-07823
Species: Hu
Applications: WB

Publications for ATP synthase C mature Lysate (NBL1-07823) (0)

There are no publications for ATP synthase C mature Lysate (NBL1-07823).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ATP synthase C mature Lysate (NBL1-07823) (0)

There are no reviews for ATP synthase C mature Lysate (NBL1-07823). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATP synthase C mature Lysate (NBL1-07823) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ATP synthase C mature Products

Bioinformatics Tool for ATP synthase C mature Lysate (NBL1-07823)

Discover related pathways, diseases and genes to ATP synthase C mature Lysate (NBL1-07823). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ATP synthase C mature Lysate (NBL1-07823)

Discover more about diseases related to ATP synthase C mature Lysate (NBL1-07823).
 

Pathways for ATP synthase C mature Lysate (NBL1-07823)

View related products by pathway.

PTMs for ATP synthase C mature Lysate (NBL1-07823)

Learn more about PTMs related to ATP synthase C mature Lysate (NBL1-07823).

Blogs on ATP synthase C mature.

Mitochondrial ATPase inhibitory factor 1 (IF1) provides an explanation of cancer growth in anoxia or pseudo-anoxia
By Jamshed Arslan Pharm.D. Adenosine triphosphate (ATP) is the major life’s energy-carrying molecule. It is mainly produced by mitochondrial ATP synthase (Complex V) through oxidative phosphorylation (Oxphos)....  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ATP synthase C mature Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ATP5MC1