ATG4D Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 647

ATG4D Antibody [Alexa Fluor™ Plus 647] Summary

• Immunogen: Antibody was raised against a 17 amino acid peptide near the amino terminus of human ATG4D. The immunogen is located within the first 50 amino acids of ATG4D. Amino Acid Squence: SPEDARRRPEARRPRG
• Specificity: At least two isoforms of ATG4D are known to exist; this antibody will detect only the larger isoform. ATG4D is predicted to not cross-react with other ATG4 proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG4D
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ATG4D Antibody [Alexa Fluor™ Plus 647]

• APG4 autophagy 4 homolog D (S. cerevisiae)
• APG4 autophagy 4 homolog D
• APG4D
• APG4-D
• ATG4 autophagy related 4 homolog D (S. cerevisiae)
• AUTL4
• AUT-like 4 cysteine endopeptidase
• AUT-like 4, cysteine endopeptidase (S. cerevisiae)
• AUT-like 4, cysteine endopeptidase
• autophagin-4
• Autophagy-related cysteine endopeptidase 4
• Autophagy-related protein 4 homolog D
• cysteine protease ATG4D
• cysteine protease involved in autophagy
• EC 3.4.22
• EC 3.4.22.-

Background

ATG4D is a gene that codes for a protein expressed in the skeletal muscle and testis and is 474 amino acids long with a weight of approximately 53 kDa. ATG4D is a cysteine protease required for autophagy. ATG4D has been shown to have interactions with TAF12, CASP3, CASP8, GABARAP, and UBC in pathways such as the regulation of autophagy pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG4D