Recombinant Human ATG4B Protein

SDS-Page: Recombinant Human ATG4B Protein [NBP1-99088] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Discontinued

Recombinant Human ATG4B Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-393 of Human ATG4B
  
  

  Source: E.coli

  Amino Acid Sequence: MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE LVEQQPSHLA CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSLLEHHHHH H

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ATG4B
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 45.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 0.1 mM PMSF
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human ATG4B Protein

• APG4 autophagy 4 homolog B (S. cerevisiae)
• APG4 autophagy 4 homolog B
• Apg4B
• ATG4 autophagy related 4 homolog B (S. cerevisiae)
• ATG4B
• AUTL1
• AUTL1MGC1353
• AUT-like 1 cysteine endopeptidase
• autophagin-1
• Autophagy-related cysteine endopeptidase 1
• Autophagy-related protein 4 homolog B
• cysteine protease ATG4B
• DKFZp586D1822
• EC 3.4.22
• EC 3.4.22.-
• hAPG4B
• KIAA0943

Background

ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ATG4B