ATG4B Antibody

Product Discontinued

ATG4B Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human ATG4B. The immunogen region is: Internal.
• Specificity: ATG4B Antibody detects endogenous levels of total ATG4B protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000

Reactivity Notes

Human,Mouse

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATG4B Antibody

• APG4 autophagy 4 homolog B (S. cerevisiae)
• APG4 autophagy 4 homolog B
• Apg4B
• ATG4 autophagy related 4 homolog B (S. cerevisiae)
• ATG4B
• AUTL1
• AUTL1MGC1353
• AUT-like 1 cysteine endopeptidase
• autophagin-1
• Autophagy-related cysteine endopeptidase 1
• Autophagy-related protein 4 homolog B
• cysteine protease ATG4B
• DKFZp586D1822
• EC 3.4.22
• EC 3.4.22.-
• hAPG4B
• KIAA0943

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.; ENZYME REGULATION: Inhibited by N-ethylmaleimide; SUBCELLULAR LOCATION: Cytoplasm;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG4B
• Uniprot:
  • Q9Y4P1(Human)