ATG3/APG3 Antibody [Alexa Fluor® 594]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

ATG3/APG3 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human ATG3
  Met1-His287
  Accession # Q9NT62
• Specificity: Detects endogenous human, mouse and rat ATG3 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

ATG3 (autophagy-related protein 3; also APG3-like and PC3-96) is a ubiquitous 45 kDa member of the ATG3 family of proteins. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG7-bound ATG8 (known as LC3, GATE16 and GABA-RAP in mammals) to phosphatidylethanolamine, critical for autophagy. Human ATG3 is 314 amino acids in length and contains an active site at Cys264 that forms a thiol ester bond with the C-terminal Gly of ATG8. There are multiple potential isoform variants. Three show a 22, 28 and 35 aa substitution for aa 289‑314, respectively, while a fourth shows an alternate start site at Met88 that may be accompanied by one of the afore mentioned substitutions. Over aa 1‑287, human ATG3 shares 97.9% aa identity with mouse ATG3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.