ATG3/APG3 Antibody (JU00-35)

Western Blot: ATG3/APG3 Antibody (JU00-35) [NBP2-75414] - Analysis of Apg3 on different lysates using anti-Apg3 antibody at 1/500 dilution.Positive control:Lane 1: Mouse testis tissue Lane 2: K562 Lane 3: HL-60 Lane 4: Hela Lane 5: Jurkat

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (JU00-35) [NBP2-75414] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Apg3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (JU00-35) [NBP2-75414] - Analysis of paraffin-embedded human tonsil tissue using anti-Apg3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (JU00-35) [NBP2-75414] - Analysis of paraffin-embedded human thyroid gland tissue using anti-Apg3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATG3/APG3 Antibody (JU00-35) [NBP2-75414] - Analysis of paraffin-embedded human colon cancer tissue using anti-Apg3 antibody. Counter stained with hematoxylin.

Product Discontinued

ATG3/APG3 Antibody (JU00-35) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ATG3/APG3 1-50 / 314. (SwissProt: Q9NT62 Human; SwissProt: Q9CPX6 Mouse)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATG3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ATG3/APG3 Antibody (JU00-35)

• APG3 autophagy 3-like (S. cerevisiae)
• APG3
• APG3L
• APG3-LIKE
• Apg3p
• ATG3 autophagy related 3 homolog (S. cerevisiae)
• ATG3
• Autophagy-related protein 3
• DKFZp564M1178
• EC 6.3.2.-
• FLJ22125,2610016C12Rik
• hApg3
• MGC15201
• PC3-96
• Protein PC3-96
• ubiquitin-like-conjugating enzyme ATG3

Background

FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoformsproduced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG3