ATG13 [p Ser318] Antibody

Images

 
Western Blot: ATG13 [p Ser318] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATG13 [p Ser318] Antibody Summary

Immunogen
Synthetic peptide corresponding to the region near S318 of ATG13. Protein accession number NP_001136145.1. The target domain of reactivity is Internal.
Modification
p Ser318
Specificity
This antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue. NBP2-19127 was cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:25000-1:175000
  • Flow Cytometry 1:1:10 - 1:1000
Application Notes
Tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human phosphorylated ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Publications
Read Publication using NBP2-19127.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATG13 [p Ser318] Antibody

  • autophagy-related protein 13
  • autophagy related 13
  • KIAA0652

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATG13 Antibody (NBP2-19127)(1)


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Secondary Antibodies

 

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ATG11 - An important scaffolding protein in autophagosome formation and fusion
Autophagy is a cellular mechanism used to regulate cell metabolism and to recycle or degrade damaged organelles and proteins. This is accomplished through the engulfment of cargo in a double-membrane structure called the autophagosome. The autophag...  Read full blog post.

ULK1 - mammalian homologue of the yeast ATG1 kinase
Autophagy is an important cellular process involved in degradation and recycling of cellular macromolecules in response to stress or starvation. Autophagy is carried out in four main phases: phagophore nucleation, autophagosome elongation, docking...  Read full blog post.

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Bioinformatics

Gene Symbol ATG13
Uniprot