ATF5 Antibody

Immunohistochemistry-Paraffin: ATF5 Antibody [NBP1-89435] - Staining of human duodenum shows moderate nuclear positivity in glandular cells.

Product Discontinued

ATF5 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PLAPYEVLGGALEGGLPVGGEPLAGDGFSDWMTERVDFTALLPLEPPLPPGTLPQPSPTPPDLEAMASLLKKELEQMEDFFLDAPPLPPPSPPPLPPPPLPPAPSLPLSLPSFDLPQPPVLDTLDL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATF5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ATF5 Antibody

• activating transcription factor 5FLJ34666
• ATFXHMFN0395
• cAMP-dependent transcription factor ATF-5
• cyclic AMP-dependent transcription factor ATF-5
• Transcription factor ATFx

Background

Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATF5 Antibody (NBP1-89435)(1)

Publication using NBP1-89435

• Vicari L, La Rosa C, Forte S et al. Differential expression of two activating transcription factor 5 isoforms in papillary thyroid carcinoma. Onco Targets Ther 2016-10-27 [PMID: 27785070] (IHC-P)

Bioinformatics

• Gene Symbol: ATF5