Human ATF2 ELISA Kit (Colorimetric)

ELISA: Human ATF2 ELISA Kit (Colorimetric) [NBP2-69881] - Samples were spiked with high concentrations of Human ATF2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human ATF2 ELISA Kit (Colorimetric) [NBP2-69881] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human ATF2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human ATF2 in samples. No significant cross-reactivity or interference between Human ATF2 and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.28%
• Intra-Assay: CV% < 4.69%
• Spike Recovery: 89-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ATF2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ATF2 ELISA Kit (Colorimetric)

• activating transcription factor 2formerly
• ATF2
• cAMP responsive element binding protein 2
• cAMP-responsive element-binding protein 2
• CREB2
• CREBP1
• CRE-BP1
• cyclic AMP-dependent transcription factor ATF-2
• HB16
• MGC111558
• mXBP
• TREB7

Background

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ATF2