ATF1 Antibody (3E7) - Azide and BSA Free

Western Blot: ATF1 Antibody (3E7) [H00000466-M03] - Analysis of ATF1 expression in transfected 293T cell line by ATF1 monoclonal antibody (M03), clone 3E7. Lane 1: ATF1 transfected lysatE (29.2 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: ATF1 Antibody (3E7) [H00000466-M03] - Analysis of monoclonal antibody to ATF1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml

Western Blot: ATF1 Antibody (3E7) [H00000466-M03] - Western blot analysis of ATF1 over-expressed 293 cell line. Lane 2 cotransfected with ATF1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000466-M03. GAPDH (36.1 kDa) used as loading control.

Sandwich ELISA: ATF1 Antibody (3E7) [H00000466-M03] - Detection limit for recombinant GST tagged ATF1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, KD
• Clone: 3E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATF1 Antibody (3E7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ATF1 (AAH29619, 1 a.a. ~ 271 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV
• Specificity: ATF1 - activating transcription factor 1 (3E7)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATF1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for RNAi Validation and IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATF1 Antibody (3E7) - Azide and BSA Free

• activating transcription factor 1FUS/ATF-1
• ATF1
• cAMP-dependent transcription factor ATF-1
• EWS-ATF1
• Protein TREB36
• TREB36
• TREB36cyclic AMP-dependent transcription factor ATF-1

Background

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF1
• Entrez:
  • 466(Human)
• Uniprot:
  • AAH29619(Human)