ATAD3B Antibody - Azide and BSA Free

Western Blot: ATAD3B Antibody [H00083858-B01P] - Analysis of ATAD3B expression in human colon.

Immunocytochemistry/Immunofluorescence: ATAD3B Antibody [H00083858-B01P] - Analysis of purified antibody to ATAD3B on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: ATAD3B Antibody [H00083858-B01P] - Analysis of ATAD3B expression in transfected 293T cell line by ATAD3B polyclonal antibody. Lane 1: ATAD3B transfected lysate(71.28 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATAD3B Antibody - Azide and BSA Free Summary

• Immunogen: ATAD3B (AAH02542.1, 1 a.a. - 648 a.a.) full-length human protein. MSWLFGVNKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKEALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQRLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVETEARARAKAERENADIIREQIRLKASEHRQTVLESIRTAGTLFGEGFRAFVTDRDKVTATVAGLTLLAVGVYSAKNATAVTGRFIEARLGKPSLVRETSRITVLEALRHPIQVSRRLLSRPQDVLEGVVLSPSLEARVRDIAIATRNTKKNRGLYRHILLYGPPGTGKTLFAKKLALHSGMDYAIMTGGDVAPMGREGVTAMHKLFDWANTSRRGLLLFMDEADAFLRKRATEEISKDLRATLNAFLYHMGQHSNKFMLVLASNLPEQFDCAINSRIDVMVHFDLPQQEERERLVRLHFDNCVLKPATEGKRRLKLAQFDYGRKCSEVARLTEGMSGREIAQLAVSWQATAYASKDGVLTEAMMDACVQDAVQQYRQKMRWLKAEGPGRGVEHPLSGVQGETLTSWSLATDPSYPCLAGPCTFRICSWMGTGLCPGPLSPRMSCGGGRPFCPPGHPLL
• Specificity: ATAD3B - ATPase family, AAA domain containing 3B,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: ATAD3B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It is also useful for immunofluoresence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATAD3B Antibody - Azide and BSA Free

• AAA-ATPase TOB3
• ATPase family AAA domain-containing protein 3B
• ATPase family, AAA domain containing 3B
• KIAA1273TOB3AAA-TOB3

Background

ATAD3A (MIM 612316) and ATAD3B are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al., 2007 [PubMed 17210950]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATAD3B Antibody (H00083858-B01P)(3)

Publications using H00083858-B01P

• Paramithiotis E, Prentki M, Rabasa-lhoret R et al. Type 2 Diabetes Biomarkers and Uses Thereof. United States Patent Application 2015-11-19
• Ann F, Alison C, Yoshihito K et al. Fatal perinatal mitochondrial cardiac failure caused by recurrent de novo duplications in the ATAD3 locus. Med (N Y). 2020-07-09 [PMID: 33575671]
• Federspiel JD, Codreanu SG, Palubinsky AM et al. Assembly dynamics and stoichiometry of the apoptosis signal-regulating kinase (ASK) signalosome in response to electrophile stress Mol. Cell Proteomics 2016-03-22 [PMID: 27006476] (WB, Human)

Bioinformatics

• Gene Symbol: ATAD3B
• Entrez:
  • 83858(Human)
• Uniprot:
  • AAH02542.1(Human)