ATAD2 Antibody (CL0182) - BSA Free

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining in human testis and skeletal muscle tissues. Corresponding ATAD2 RNA-seq data are presented for the same tissues.

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of MCF7 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human rectum shows strong nuclear positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786] - Simple Western lane view shows a specific band for ATAD2 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.

Simple Western: ATAD2 Antibody (CL0182) [NBP2-14786] - Electropherogram image(s) of corresponding Simple Western lane view. ATAD2 antibody was used at 1:20 dilution on U-251MG sp lysates(s).

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC, Mycoplasma
• Clone: CL0182
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ATAD2 Antibody (CL0182) - BSA Free Summary

• Description: Novus Biologicals Mouse ATAD2 Antibody (CL0182) - BSA Free (NBP2-14786) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q6PL18, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATAD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Simple Western 1:20
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp, separated by Size, antibody dilution of 1:20, apparent MW was 182 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  ATAD2 Recombinant Protein Antigen (NBP2-14786PEP)

Reactivity Notes

Expected species cross reactivity based on sequence identity: Mouse( 82%), Rat (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for ATAD2 Antibody (CL0182) - BSA Free

• AAA nuclear coregulator cancer-associated protein
• ANCCA
• ANCCAMGC131938
• ATAD2
• ATPase family AAA domain-containing protein 2
• ATPase family, AAA domain containing 2
• CT137
• DKFZp667N1320
• EC 3.6.1.3
• MGC142216
• MGC29843
• MGC5254
• PRO2000

Background

ATAD2 is a large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATAD2