ATAD2 Antibody (CL0182) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: ATAD2 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) Lane 3: Negative control (vector only transfected HEK293T lysate)Lane 4: U-251

Analysis in human testis and skeletal muscle tissues using NBP3-44437 antibody. Corresponding ATAD2 RNA-seq data are presented for the same tissues.

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Staining of human rectum shows strong nuclear positivity in a subset of glandular cells.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of MCF7 cells using the Anti-ATAD2 monoclonal antibody) .

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: CL0182
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ATAD2 Antibody (CL0182) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q6PL18, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATAD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Simple Western 1:20
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
  Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.
  See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp, separated by Size, antibody dilution of 1:20, apparent MW was 182 kDa

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ATAD2 Antibody (CL0182) - Azide and BSA Free

• AAA nuclear coregulator cancer-associated protein
• ANCCA
• ANCCAMGC131938
• ATAD2
• ATPase family AAA domain-containing protein 2
• ATPase family, AAA domain containing 2
• CT137
• DKFZp667N1320
• EC 3.6.1.3
• MGC142216
• MGC29843
• MGC5254
• PRO2000

Background

ATAD2 is a large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATAD2