Aspartate Aminotransferase Antibody (3G11) - BSA Free

Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2

Immunocytochemistry/Immunofluorescence: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Analysis of GOT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of GOT1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Lane 1: 293T cell lysates, Lane 2: GOT1 Transfected 293T cell lysates

Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : A427 cell lysate

Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Aspartate Aminotransferase Antibody (3G11) - BSA Free Summary

• Description: Novus Biologicals Mouse Aspartate Aminotransferase Antibody (3G11) - BSA Free (NBP2-59483) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Aspartate Aminotransferase (1-413aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1080
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Aspartate Aminotransferase Antibody (3G11) - BSA Free

• aspartate aminotransferase, cytoplasmic
• AST1
• ASTQTL1
• CASPAT
• CCAT
• Cysteine aminotransferase, cytoplasmic
• Cysteine transaminase, cytoplasmic
• EC 2.6.1.1
• EC 2.6.1.3
• GIG18
• Glutamate oxaloacetate transaminase 1
• glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
• GOT1
• growth-inhibiting protein 18
• SGOT
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOT1