ASH2L Antibody

Images

 
Western Blot: ASH2L Antibody [NB600-253] - Whole cell lysate (A. 40 ug - E; 10 ug - T) from 293T cells that were mock transfected (E) or transfected with an ASH2 expression construct (T). Antibody used at the ...read more
Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-253] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ASH2L Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 628) of human Absent, Small, or Homeotic-Like 2 using the numbering given in entry NP_004665.1 (GeneID 9070).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASH2L
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 to 1:10,000 Immunoprecipitation: NB 600-253 works well in IP for mouse. For human, use NB 600-250. Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Positive Control
ASH2L Lysate (NBL1-07765)
Publications
Read Publication using NB600-253.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASH2L Antibody

  • ash2 (absent, small, or homeotic)-like (Drosophila)
  • ASH2
  • ASH2L1ash2 (absent, small, or homeotic, Drosophila, homolog)-like
  • ASH2L2ASH2-like protein
  • Bre2
  • set1/Ash2 histone methyltransferase complex subunit ASH2

Background

The ASH2 gene is a member of the trithorax group of genes whose products function to maintain active transcription of homeotic selector genes. Mutations in ash2 cause the homeotic transformations expected for a gene in this group but, in addition, cause a variety of pattern formation defects that are not necessarily expected. The ash2 gene is located in cytogenetic region 96A17-19 flanked by slowpoke and tolloid and is included in a cosmid that contains part of slowpoke. Ash2, the product of the Drosophila trxG gene absent, small, or homeotic discs 2 (ash2) is a component of a 500-kD complex. ASH2 binds directly to Skittles (Sktl), a predicted phosphatidylinositol 4-phosphate 5-kinase, and the association of these proteins is functionally significant. Histone H1 hyperphosphorylation is dramatically increased in both ash2 and sktl mutant polytene chromosomes. These results suggest that Ash2 maintains active transcription by binding a producer of nuclear phosphoinositides and downregulating histone H1 hyperphosphorylation. Both Ash2 and Sktl accumulate on polytene chromosomes and in the nucleolus. These results suggest that their physical association and functions involve chromatin and, perhaps, ribosomal DNA transcription. Ash2 accumulates normally on sktl mutant chromosomes while Sktl does not accumulate on ash2 mutant chromosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASH2L Antibody (NB600-253)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ASH2L
Entrez
Uniprot