Artemis Antibody

Images

 
Western Blot: Artemis Antibody [NBP1-77975] - Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.
Immunohistochemistry: Artemis Antibody [NBP1-77975] - Used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.2 mg/ml

Order Details

Artemis Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 482-495 of Human ARTEMIS (DCLRE1C DNA cross-link repair 1C).
Specificity
This antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express artemis, such as the cell line CJ (see figure) and in an hTERT line (not shown).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCLRE1C
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000-1:20000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
This antibody detects a band of approximately 90 kDa.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Artemis Antibody

  • ASCID
  • DCLREC1C
  • DNA cross-link repair 1C protein
  • DNA cross-link repair 1C
  • EC 3.1
  • FLJ36438
  • hSNM1C
  • protein artemis
  • Protein A-SCID
  • PSO2 homolog
  • RS-SCID
  • severe combined immunodeficiency, type a (Athabascan)
  • SNM1 homolog C
  • SNM1CS. cerevisiae)
  • SNM1-like protein

Background

Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DCLRE1C
Uniprot